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Vitamin B2 Test (Riboflavin Test)

This Vitamin B2 Test, sometimes referred to as a riboflavin test, is used to measure the amount of vitamin B2 (riboflavin) in the blood.

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Test Preparation:
Fasting is required (i.e. no eating or drinking anything besides water for 8 hours prior to testing)
Testing Method:
Blood draw

This Vitamin B2 Test, sometimes referred to as a riboflavin test, is used to measure the amount of vitamin B2 (riboflavin) in the blood. Vitamin B2 is responsible for energy production and enzyme function as well as fatty and amino acid synthesis. Those with vitamin B2 deficiencies are subject to tongue sores, chapped lips, and scaly skin rashes. Vitamin B2 deficiencies can be reliably identified with this Vitamin B2 Test and are most common in those with kidney disease, hyperthyroidism, cirrhosis, or those who drink heavily.

Vitamin B2 (Riboflavin) Test

For our Vitamin B2 (Riboflavin) test, simply place your order online and stop by one of our 4000+ nationwide testing facilities at your convenience (no appointments). A member of the center’s staff will collect a small blood sample via the skin prick method, at which point your part of the process is done. 

The sample collection itself takes only a few minutes, and most patients are in and out and about their day in under half an hour. From there, your sample will be processed in a CLIA-certified laboratory, and in as little as 24-72 hours, you’ll have full results regarding your possible Vitamin B2 deficiency.

Common Vitamin B2 (Riboflavin) Deficiency Symptoms

Vitamin B2, also known as Riboflavin, is one of eight B vitamins essential for human health. Riboflavin is water-soluble and essentially nontoxic. Vitamin B2 plays a key role in the metabolism of fats, proteins, and carbohydrates into glucose for energy. 

Being a water-soluble vitamin, Riboflavin passes through the bloodstream, and whatever the body does not need, it passes out via urine. The body can only store small amounts of vitamin B2, which it goes through quickly. As a result, we need to consume vitamin B2 every day. 

Dietary sources of vitamin B2 include egg, meat, liver, cheese, and milk. An inadequate supply of vitamin B2 in your diet or your body’s inability to absorb the nutrient can lead to vitamin B2 deficiency. Common symptoms of Riboflavin deficiency include: 

  • Dry skin
  • Cracked lips
  • Cracks at the corners of the mouth
  • Red lips
  • Ulcers in the mouth
  • Fluid in mucous membranes
  • Scrotal dermatitis

If you are experiencing these symptoms, your doctor may order a vitamin B2 test to rule out Riboflavin deficiency. 

How to Interpret Your Results

Our Vitamin B2 (Riboflavin) test is a blood test that checks the levels of Vitamin B2 in the body. Low vitamin B2 levels in the blood indicate deficiency. Your healthcare provider may order more tests to determine whether your B vitamin deficiency is due to your body’s inability to absorb or use B vitamins or inadequate supply.

The results can also come back normal; if they do, something else is causing your symptoms. The test may also show high vitamin B2 in the bloodstream, a phenomenon associated with vitamin toxicity. This rare diagnosis is attributable to exposure to high doses of Riboflavin. 

Our Vitamin B2 test will indicate whether your Riboflavin levels fall above or below the interval of values deemed normal for healthy persons or the reference range. You can find the outcome of the test under the ‘flag’ column of the results chart. Here, the Vitamin B2 in your blood will be reported as either normal or abnormal. 

What Are the Possible Complications of Vitamin B2 (Riboflavin) Deficiency?

Severe vitamin B2 deficiency can lead to serious health complications. For instance, low levels of Riboflavin in the body can alter iron absorption and cause anemia. Anemia is a condition resulting from the lack of enough healthy red blood cells. Although most cases are mild, anemia can be severe and life-threatening.


  • A vitamin B2 deficiency usually stems from inadequate amounts of this essential micronutrient in the diet or an absorption disorder. Riboflavin is naturally present in grains, plants, and dairy products. 

  • The best way to measure Vitamin B2 levels is via a blood test. All you have to do for our Vitamin B2 test is place your order online and stop by one of our testing facilities to have your blood sample taken. 

  • The amounts of vitamin B2 in your body can also be too high. Very high levels of Riboflavin can cause sensitivity to light, yellow or orange urine, burning or prickling, numbness, and itching.